Pine Kinesin

Pine (Pinus Taeda) Kinesins
A tblastn search on TIGR was run against the pine genome with the FASTA format of the kinesin heavy chain sequence of Drosophila melanogaster (P17210). This was done to determine the number of kinesin in pine trees. The FASTA format of the protein sequence was found using the acession number as a query in Entrez Protein. A total of 20 hits were found with decent scores and E values. However, upon further analysis of each sequence several were found to not be kinesins. For example, one protein was found to be a neurofilament protein. In general, the TIGR blast website provided several benefits over the NCBI blast website. TIGR blast allowed the search of more specific organism genomes, even incomplete genomes. For example, NCBI did not have the pine genome in its database, while TIGR allowed a query search against the pine genome. The result format was similar to NCBI blast results. TIGR blast even had links to the proteins on NCBI. One setback of TGIR blast was the slow execution of the blast search. Some searches took several minutes compared to the 15 seconds in NCBI blast.

The total Database: Pine 45,557 sequences; 36,548,862 total letters

pineDR691181 similar to UPQ69UI4 (Q69UI4) Kinesin 1-like
Score 409 E value 1.6e-51
pineTC61066 similar to UPQ9M0X6 (Q9M0X6) Kinesin-like protein
Score 389 E value 2.4e-35
pineTC62224 similar to UPATK1_ARATH (Q07970) Kinesin 1
Score 327 E value 1.5e-28
pineDR080983 similar to GBBAB09933.110176794AB022214 kinesin-like protein
Score 275 E value 7.7e-23
pineBM157420 similar to UPQ9LXV6 (Q9LXV6) Kinesin-like protein
Score 185 E value 3.9e-22
pineTC67370 similar to UPQ6WJ05 (Q6WJ05) Central motor kinesin 1
Score 258 E value 1.1e-18
pineCF666088 similar to UPQ75UP8 (Q75UP8) Kinesin heavy chain-like protein
Score 202 E value 1.7e-17
pineDR182269 homologue to UPQ6WJ05 (Q6WJ05) Central motor kinesin 1
Score 178 E value 3.3e-11
pineDR684708 similar to UPQ6YZ52 (Q6YZ52) Kinesin motor protein 1-like
Score 176 E value 4.5e-11
pineDR100363 UPKINH_GIBMO (Q86Z98) Kinesin heavy chain
Score 152 E value 1.3e-09
pineBF610071 similar to GBBAB19066.111761076AP002744 kinesin-like protein
Score 114 E value 2.4e-05