Plant Kinesin Lab 2

Vitis vinifera (grape) Kinesin!

• grapeTC48382
  Kinesin-like calmodulin binding protein, partial
  Score: 362; E value: 1.2e-32
• grapeTC43494
  Kinesin-like protein KIFC3, partial
  Score: 311; E value: 4.6e-31
• grapeTC39410
  Central motor kinesin 1, partial
  Score: 363; 9.2e-31
• grapeTC44010
  Kinesin-related protein-like, partial
  Score: 210; E value: 3.7e-16

I tried several different methods to located the Vitis vinifera kinesin before I found a way that worked. I have to admit I did try googling "grape kinesin" but alas didn't yeild any helpful results. Also tried browsing the NCBI site as that worked for some other groups but didn't really find anything helpful there either. I browsed the TIGR site for a while and tried doing searches with part of the nucleotide sequence for the P17210 protein (to only capture the head region) and the whole nucleotide sequence. No luck there. Also tried doing part of the protein sequence but eventually found that performing a tblastn search on the TIGR database using the whole protein sequence (which I retrieved from FASTA) gave me the best results. I did have to analyze the results and select the proteins that were most related to the head region (1st 350 amino acids). I had to do this rather than copy and paste the results because some of the heavy chain areas had higher similarity scores than the head region and therefore were mixed in my main results. I also found that some of the results were a repeated protein. Other than that I'd have to say that the TIGR website was a good resource for looking for a specific specie's kinesin. I would like to learn more about it in terms of how to limit the parameters and manipulate the results a little more.